Curriculum Vitaes

Atsuko Takagi

  (高木 敦子)

Profile Information

Affiliation
University Research Administrator, Osaka Sangyo University
Degree
Doctor of Engineering(Jul, 1985)
大阪大学工学修士(Mar, 1981, 大阪大学)
奈良女子大学理学士(Mar, 1979, 奈良女子大学)

J-GLOBAL ID
200901049532549229
researchmap Member ID
0000010994

Papers

 50
  • Ming Li, Ken-Ichi Hirano, Yoshihiko Ikeda, Masahiro Higashi, Chikako Hashimoto, Bo Zhang, Junji Kozawa, Koichiro Sugimura, Hideyuki Miyauchi, Akira Suzuki, Yasuhiro Hara, Atsuko Takagi, Yasuyuki Ikeda, Kazuhiro Kobayashi, Yoshiaki Futsukaichi, Nobuhiro Zaima, Satoshi Yamaguchi, Rojeet Shrestha, Hiroshi Nakamura, Katsuhiro Kawaguchi, Eiryu Sai, Shu-Ping Hui, Yusuke Nakano, Akinori Sawamura, Tohru Inaba, Yasuhiko Sakata, Yoko Yasui, Yasuyuki Nagasawa, Shintaro Kinugawa, Kazunori Shimada, Sohsuke Yamada, Hiroyuki Hao, Daisaku Nakatani, Tomomi Ide, Tetsuya Amano, Hiroaki Naito, Hironori Nagasaka, Kunihisa Kobayashi
    Orphanet journal of rare diseases, 14(1) 134-134, Jun 11, 2019  Peer-reviewed
    Triglyceride deposit cardiomyovasculopathy (TGCV) is a phenotype primarily reported in patients carrying genetic mutations in PNPLA2 encoding adipose triglyceride lipase (ATGL) which releases long chain fatty acid (LCFA) as a major energy source by the intracellular TG hydrolysis. These patients suffered from intractable heart failure requiring cardiac transplantation. Moreover, we identified TGCV patients without PNPLA2 mutations based on pathological and clinical studies. We provided the diagnostic criteria, in which TGCV with and without PNPLA2 mutations were designated as primary TGCV (P-TGCV) and idiopathic TGCV (I-TGCV), respectively. We hereby report clinical profiles of TGCV patients. Between 2014 and 2018, 7 P-TGCV and 18 I-TGCV Japanese patients have been registered in the International Registry. Patients with I-TGCV, of which etiologies and causes are not known yet, suffered from adult-onset severe heart disease, including heart failure and coronary artery disease, associated with a marked reduction in ATGL activity and myocardial washout rate of LCFA tracer, as similar to those with P-TGCV. The present first registry-based study showed that TGCV is an intractable, at least at the moment, and heterogeneous cardiovascular disorder.
  • Ken-ichi Hirano, Masahiro Higashi, Hideyuki Miyauchi, Atsuko Takagi, Yasuyuki Ikeda, Yusuke Nakano, Tetsuya Amano, for, the Japan TGCV, Study Group
    Annals of Nuclear Cardiology, accepted(1) 47-49, 2019  Peer-reviewed
  • Atsuko Takagi, Yasuyuki Ikeda, Kunihisa Kobayashi, Kazuhiro Kobayashi, Yoshihiko Ikeda, Junji Kozawa, Hideyuki Miyauchi, Ming Li, Chikako Hashimoto, Yasuhiro Hara, Satoshi Yamaguchi, Akira Suzuki, Tatsushi Toda, Hironori Nagasaka, Ken-ichi Hirano
    Biochemical and Biophysical Research Communications, 495(1) 646-651, Jan 1, 2018  Peer-reviewedLead author
    Triglyceride deposit cardiomyovasculopathy (TGCV) is a rare and newly identified disease among patients requiring cardiac transplantation. TGCV is characterized by cardiomyocyte steatosis and triglyceride (TG)-deposit atherosclerosis, resulting from the abnormal intracellular metabolism of TG. TGCV is classified into primary and idiopathic types. Primary TGCV carries ultra-rare genetic mutations in the adipose triglyceride lipase (ATGL), a rate-liming enzyme that hydrolyzes intracellular TG in adipose and non-adipose tissues. Idiopathic TGCV, first identified among autopsied individuals with diabetes mellitus (DM) with severe heart diseases, shows no ATGL mutations and its causes and underlying mechanisms are still unknown. TGCV is difficult to diagnose in daily clinics, thereby demanding feasible diagnostic procedures. We aimed to develop an assay to measure ATGL activity using peripheral leucocytes. Human his6-ATGL was expressed in COS1 cells, purified to homogeneity, and used to raise a polyclonal antibody neutralizing TG-hydrolyzing activity of ATGL. We developed a selective immunoinactivation assay (SIIA) for the quantitation of ATGL activity in cell lysates of leucocytes by the antibody neutralizing ATGL activities. ATGL activity was measured in 13 idiopathic TGCV patients, with two patients with primary TGCV as the negative control. Healthy (non-DM) and DM controls without heart diseases were also subjected. The developed SIIA assay revealed significant reduction in ATGL activity in leucocytes from patients with idiopathic TGCV who did not carry ATGL mutations as compared with non-DM and DM controls. Thus, ATGL in leucocytes may be an important biomarker for the diagnosis of TGCV and our assay may provide insights into pathophysiology and elucidate the underlying mechanism of TGCV and related disorders.
  • Akira Suzuki, Satoshi Yamaguchi, Ming Li, Yasuhiro Hara, Hideyuki Miyauchi, Yoshihiko Ikeda, Bo Zhang, Masahiro Higashi, Yasuyuki Ikeda, Atsuko Takagi, Hironori Nagasaka, Kunihisa Kobayashi, Yasuhiro Magata, Toshiaki AoyamaI, Ken-ichi Hirano
    J Oleo Science, 67(8) 983-989, 2018  Peer-reviewed
  • Sawa Minohara, Sung Kwan Bae, Saori Sugiyama, Noriko Shibata, Toshifumi Gushima, Junichi Motoshita, Shinji Shimoda, Atsuko Takagi, Yasuyuki Ikeda, Kazuhiro Takahashi
    Clinical Case Reports, 6(9) 1-5, 2018  Peer-reviewed
    We report a case of non-alcoholic steatohepatitis complicated with acute pancreatitis induced by hypertriglyceridemia in a young Japanese woman. A precise examination of the lipid profile showed decreased lipoprotein lipase (LPL) and hepatic triglyceride lipase activity levels, while the LPL mass was at the minimum level of the normal range.
  • Atsuko Takagi, Yasuyuki Ikeda
    Nihon rinsho. Japanese journal of clinical medicine, 71(9) 1569-76, Sep, 2013  
    Human LPL is a glycoprotein enzyme with a molecular mass of 61 kDa, and it plays a key role in regulating the triglyceride (TG) levels in circulation by hydrolyzing TGs in TG-rich lipoproteins at the first step in their metabolism. Homozygous or compound heterozygous LPL deficiency causes severe fasting hypertriglyceridemia. Heterozygous LPL deficiency usually results in a normolipidemic state, but this may cause mild hypertriglyceridemia if heterozygotes are exposed to factors, such as high alcohol intake and/or a hyperinsulinemic state. Severe fasting hypertriglyceridemia is mainly caused by abnormalities of the LPL gene, whereas there are some cases caused by gene defects relating to synthesis and transport of LPL such as LMF and GPIHBP1, and by an autoantibody to LPL acting as inhibitor of LPL.
  • Atsuko Takagi, Yasuyuki Ikeda
    Nihon rinsho. Japanese journal of clinical medicine, 65 Suppl 7 182-90, Jul 28, 2007  
  • 池田康行, 高木敦子, 西村 誠, 大軽靖彦
    医学と薬学, 57 737-742, 2007  Peer-reviewed
  • N Tamasawa, J Matsui, H Murakami, J Tanabe, K Matsuki, Y Ogawa, Y Ikeda, A Takagi, T Suda
    DIABETES RESEARCH AND CLINICAL PRACTICE, 72(1) 6-11, Apr, 2006  Peer-reviewed
    Elevations in plasma triglyceride (TG) and free fatty acid (FFA) concentrations are generally thought to play a role in the pathogenesis of insulin-resistant diabetes. The objective of this study was to investigate the relationship between hypertriglyceridemia and glucose-stimulated insulin responsiveness in non-diabetic patients. Forty subjects were divided into three BMI-matched groups as follows: one group consisted of 8 patients with a lipoprotein lipase (LPL) deficiency, another consisted of 12 patients with hypertriglyceridemia and a third consisted of 20 subjects with normal TG levels. In response to a 75 g oral glucose tolerance test, plasma insulin levels in the LPL-deficient subjects were higher (106 +/- 11 mu U/ml) than those in the hypertriglyceridemic (69 +/- 16 mu U/ml) and normolipidemic (29 3 mu U/ml) subjects, at 30 min. On the other hand, their plasma glucose levels (127 +/- 6 mg/dl) were less than those seen in the normolipidemic group (165 +/- 9 mg/dl) after 90 min. Thus, LPL-deficient subjects with hypertriglyceridemia displayed an enhanced glucose-stimulated insulin response as well as lower blood glucose levels, the latter of which is not generally seen in those with hypertriglyceridemia and normolipidemia. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
  • Koichi Ono, Mitsuyasu Koike, Takuhito Ohse, Atsuko Takagi, Yasuyuki Ikeda
    2006 INTERNATIONAL CONFERENCE ON MICROTECHNOLOGIES IN MEDICINE AND BIOLOGY, 198-+, 2006  Peer-reviewed
    Temperature-controlled on-chip capillary electrophoresis (CE) was applied to multi-temperature single-strand conformation polymorphism (SSCP) analysis for the detection of three lipoprotein lipase (LPL) gene mutations. In the SSCP analysis, five different temperature conditions (10, 15, 20, 25, and 30 degrees C) were used to obtain an SSCP pattern. Differences between the electropherograms of Cy5-labeled PCR products of wild/wild homozygote and wild/mutant heterozygote were clearly detected for all four LPL mutations in at least one appropriate temperature condition. These results indicate that our SSCP system is applicable to high-throughput and accurate SSCP screening for locating unknown as well as known single nucleotide polymorphisms (SNPs).
  • M Nishimura, T Iwanaga, Y Ohkaru, A Takagi, Y Ikeda
    JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY, 27(1) 89-102, 2006  Peer-reviewed
    Objectives of this work are to study changes in the immunoreactive HTGL mass during storage under various conditions. In addition, the shelf-life of the HTGL ELISA kit was confirmed. The immunological reactivity of HTGL in PHP stored in the liquid, frozen, or lyophilized state was monitored using purified human PHP-HTGL as the standard material. Furthermore, the long-term stability of the HTGL ELISA kit was ascertained. The immunoreactive HTGL mass in the lyophilized PHP maintained its initial immunological reactivity for at least 26 months at 4 degrees C or lower. The other reagents included in the HTGL ELISA kit also have a long shelf-life when they are stored at 4 degrees C or less. HTGL in PHP was stabilized by lyophilization and can be used as the standard material for HTGL ELISA; the HTGL ELISA kit has a long shelf-life, i.e., more than two years.
  • T Nojima, K Yamashita, A Takagi, Y Ikeda, H Kondo, S Takenaka
    ANALYTICAL SCIENCES, 21(12) 1437-1441, Dec, 2005  Peer-reviewed
    A ferrocenylnaphthalene diimide (FND)-based electrochemical hybridization assay (FND-EHA) was applied to the detection of two mutations in human lipoprotein lipase (LPL) gene, G188E (one base transition) and Arita (one base deletion). A probe oligodeoxyribonucleotide of 13 bases representing the wild type (WT) sequence of LPL was immobilized on a gold electrode, followed by hybridization with a sample PCR product of 350 base pairs under conditions in which both WT and mutated (MT) sequences could form a duplex with the probe. The hybridized electrodes were soaked in an electrolyte containing FND under conditions in which only the mismatched duplex could undergo dissociation. FND was concentrated in proportion to the amount of the duplex remaining on the electrode to,give rise to a current signal. Blind tests were run to judge the genotype (WT/WT, WT/MT, or MT/MT) of 10 samples each for the G188E and Arita mutations and then, 8 and 10 of them were judged correctly, respectively.
  • Atsuko Takagi, Yasuyuki Ikeda
    Nihon rinsho. Japanese journal of clinical medicine, 62 Suppl 12 71-8, Dec, 2004  
  • M Harada-Shiba, A Takagi, K Marutsuka, S Moriguchi, H Yagyu, S Ishibashi, Y Asada, S Yokoyama
    CIRCULATION RESEARCH, 95(9) 945-952, Oct, 2004  Peer-reviewed
    We previously characterized the patients with autosomal recessive hypercholesterolemia (ARH) as having severe hypercholesterolemia and retarded plasma low-density lipoprotein (LDL) clearance despite normal LDL receptor ( LDLR) function in their cultured fibroblasts, and we identified a mutation in the ARH locus in these patients. ARH protein is an adaptor protein of the LDL and reportedly modulates its internalization. We developed ARH knockout mice (ARH(-/-)) to study the function of this protein. Plasma total cholesterol level was higher in ARH(-/-) mice than that in wild-type mice (ARH(+/+)), being attributed to a 6-fold increase of LDL, whereas plasma lipoprotein was normal in the heterozygotes (ARH(+/-)). Clearance of I-125-LDL from plasma was retarded in ARH(-/-) mice, as much as that found in LDLR-/- mice. Fluorescence activity of the intravenously injected 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI)-LDL was recovered in the cytosol of the hepatocytes of ARH(+/+) mice, but not in those of ARH(-/-) or LDLR-/- mice. Also, less radioactivity was recovered in the liver of ARH(-/-) or LDLR-/- mice when [H-3]cholesteryl oleyl ether (CE)-labeled LDL was injected. In contrast, uptakes of [H-3]CE-labeled LDL, I-125-LDL, and DiI-LDL were all normal or slightly subnormal when the ARH(-/-) hepatocytes were cultured. We thus concluded that the function of the hepatic LDLR is impaired in the ARH(-/-) mice in vivo, despite its normal function in vitro. These findings were consistent with the observations with the ARH homozygous patients and suggested that certain cellular environmental factors modulate the requirement of ARH for the LDLR function.
  • J Wakai, A Takagi, M Nakayama, T Miya, T Miyahara, T Iwanaga, S Takenaka, Y Ikeda, M Amano
    NUCLEIC ACIDS RESEARCH, 32(18) e141, 2004  Peer-reviewed
    We describe the development of a new type of DNA array chip that utilizes electrochemical reactions and a novel method of simultaneously identifying multiple genetic mutations on an array chip. The electrochemical array (ECA) uses a threading intercalator specific to double-stranded nucleotides, ferrocenylnaphthalene diimide (FND), as the indicator. ECA does not require target labeling, and the equipment is simple, durable and less expensive. The simultaneous multiple mutation detection (SMMD) system using an ECA chip and FND utilizes an enzyme to simultaneously distinguish several genetic mutations such as single nucleotide polymorphism (SNP), insertion, deletion, translocation and short tandem repeat. We examined this SMMD system using an ECA chip, by detecting seven different mutations on the lipoprotein lipase (LPL) gene for 50 patients in a blind test. It turned out that all the results obtained were concordant with the sequencing results, demonstrating that this system is a powerful tool for clinical applications.
  • M Nishimura, T Iwanaga, Y Ohkaru, A Takagi, Y Ikeda
    YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 123(7) 587-591, Jul, 2003  Peer-reviewed
    The present study describes how to process human postheparin plasma (PHP) containing hepatic triglyceride lipase (HTGL) that is utilized as a standard material of HTGL for the quantification of HTGL mass in human plasma. The optimal storage conditions for PHP were established by monitoring the stability of HTGL molecules in PHP as an antigen, which was stored in the liquid, frozen, or lyophilized state, using purified human PHP-HTGL as the standard material and a commercial HTGL ELISA MARUPI kit, which is a direct sandwich enzyme-linked immunosorbent assay (ELISA). The HTGL ELISA MARUPI kit, for which the validity was confirmed by precision and dilution tests, showed that the immunoreactive mass of HTGL in lyophilized PHP remained stable for at least 12 months at a storage temperature of 4degreesC or lower. These results indicate that lyophilized PHP stored at a temperature of less than 4degreesC can be utilized as the standard material for the quantification of HTGL in human plasma using the HTGL ELISA MARUPI kit.
  • M Harada-Shiba, A Takagi, Y Miyamoto, M Tsushima, Y Ikeda, S Yokoyama, A Yamamoto
    JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 88(6) 2541-2547, Jun, 2003  Peer-reviewed
    Previously we have reported on siblings with severe hypercholesterolemia, xanthomas, and premature atherosclerosis without any impairment of low-density lipoprotein receptor in their fibroblasts as a first characterization of autosomal recessive hypercholesterolemia (ARH). Recently, mutations were identified for this disease in a gene encoding a putative adaptor protein. The purpose of this study was to examine the molecular pathogenesis of ARH in Japanese siblings. A novel insertion mutation was discovered in the ARH gene of the siblings. An insertion of an extra cytosine residue was identified in a locus comprising eight consecutive cytosines at positions 599 through 606 in exon 6, resulting in a sequence of nine cytosines and generating an early stop codon at 657 - 659. The mother was heterozygous for this mutation. Neither transcription product nor protein of ARH was detected in the fibroblasts of the homozygous patients. A single nucleotide polymorphism was discovered among the normal control subjects at position 604 ( cytosine to thymine: ARH-604C to ARH-604T), which changes the proline residue at 202 to serine. Interestingly, ARH is caused by a mutation of cytosine to adenine at this same position. Both siblings exhibited fatty liver, which may also be related to this mutation.
  • T Nojima, K Yamashita, A Takagi, M Takagi, Y Ikeda, H Kondo, S Takenaka
    ANALYTICAL SCIENCES, 19(1) 79-83, Jan, 2003  Peer-reviewed
    A ferrocenylnaphthalene diimide-based electrochemical hybridization assay (FND-EHA) was applied to the direct detection of a C-to-G transition in a codon (TCA) for Ser-447 of the human lipoprotein lipase (LPL) gene, which resulted in the termination of the LPL protein there. Either one of two 13-meric oligonucleotide probes, 5447 WT and S447X MT, representing sequences complementary to those of the wild type (WT) and mutated (MT) forms, was immobilized on a gold electrode, followed by hybridization with chromosomal DNA extracted from human leukocytes under the condition in which both WT- and MT-type sequences can form a duplex. These two electrodes were soaked in an electrolyte containing FND under a condition [0.1 M HOAc/KOAc (pH 5.6) containing 0.1 KCl and 0.05 mM FND at 40 degreesC], in which only the MT duplex could undergo dissociation. FND was concentrated in proportion to the amount of the duplex formed on the electrode to give rise to a current signal. The electrochemical signal ratios obtained for: WT/WT, WT/MT and MT/MT were close to the theoretical 2:1:0 with the S447 WT-modified electrode, and was again close to 0:1:2 with the S447X MT-modified one.
  • K Yamashita, A Takagi, M Takagi, H Kondo, Y Ikeda, S Takenaka
    BIOCONJUGATE CHEMISTRY, 13(6) 1193-1199, Nov, 2002  Peer-reviewed
    An electrochemical hybridization assay has been devised that enables the rapid analysis of a heterozygous deficiency of the human lipoprotein lipase (LPL) gene. PCR products of 350 base pairs (bp) containing the wild-type sequence, a mutated G(818) --> A transition or a G(916) deletion of the LPL gene were subjected to hybridization with a probe DNA of 13 or 15 bases that represented either the wild-type or the mutated sequence immobilized on a gold electrode. The differential pulse voltammetry of the electrode before and after hybridization was determined in the presence of ferrocenylnaphthalene diimide (FND) at 460 mV. The measured change in peak current, Deltai, was defined by (i - i(o))i(o) x 100%, where i. and i represent the current before and after hybridization, respectively. Matched combinations of sample and probe gave Deltai values of 40-90%, whereas mismatched combinations gave values of 20-35%, enabling the discrimination of matched hybrids from mismatched ones across a slim margin. Because the heterozygote contains both the wild-type and mutated sequences, however, it alone gives large Deltai values with both the wild- and mutant-type probes. This system was validated on 10 unknown samples of each of the two types of LPL mutation, which were correctly identified in every case.
  • 熊谷 秀規, 中村 正, 豊島 明義, 遠藤 秀彦, 高木 敦子, 池田 康行
    胆と膵, 23(9) 783-787, Sep, 2002  Peer-reviewed
    48歳男.腹痛と嘔吐を主訴とし,重症急性膵炎Stage2と判断した.習慣性飲酒歴があり44歳時に高脂血症を指摘されたが放置し,46歳時に膵炎の既往がある.入院時検査でトリグリセリド(TG)が高値であったが,入院直後は絶食とし,その後の脂肪制限食により第9病日に正常化した.血中リポ蛋白-TG水解に関与しているヘパリン静注後血漿リポプロテインリパーゼ(LPL)は一過性に減少したが,LPLは遺伝子解析で異常を認めず他に由来すると判断した.本症例の病態と成因は,習慣性過量飲酒により高TG血症と膵炎をきたし,炎症性サイトカインによりLPLが減少したため強度の高TG血症となり,さらに膵炎を増悪したと考察した
  • Y Ikeda, A Takagi, Y Nakata, Y Sera, S Hyoudou, K Hamamoto, Y Nishi, A Yamamoto
    CLINICA CHIMICA ACTA, 316(1-2) 179-185, Feb, 2002  Peer-reviewed
    Case report: A case is presented of predisposing a patient's father with obligate heterozygous lipoprotein lipase (LPL) deficiency to mild hypertriglyceridemia in Japanese I-family members (n = 8) with patient DI, who was a compound heterozygote for a novel missense mutation of G154V (GG(716)C-->GTC/Gly(154)Val) in exon 5 and a novel splice mutation (Int8/5'-dss/t( + 2)c; a T-to-C transition in the invariant GT at position + 2 of the 5' donor splice site (dss)) in intron 8 of the LPL gene. Results: The patient's father and paternal grandmother were heterozygotes for the Int8/5'-dss/t( + 2)c allele, while the patient's mother and maternal grandmother were heterozygotes for the G154V allele. These four heterozygous carriers with one defective LPL allele showed 45-57% of the mean LPL activity and mass in the post-heparin plasma (PHP) observed in normal individuals. Among the four heterozygous carriers, the patient's father, who was <40 years old, nonobese and hyperinsulinemia, manifested mild hypertriglyceridemia (type IV hyperlipoproteinemia). The remaining three healthy heterozygous carriers (two were >40 years old and the other was < 40 years old) were all normolipidemic state. Conclusion: In this family, hyperinsulinemia as a marker of insulin resistance may be a strong determinant of hypertriglyceridemia in the carrier with heterozygous LPL deficiency. (0 2002 Elsevier Science B.V. All rights reserved.
  • Y Ikeda, A Takagi, Y Nakata, Y Sera, S Hyoudou, K Hamamoto, Y Nishi, A Yamamoto
    JOURNAL OF LIPID RESEARCH, 42(7) 1072-1081, Jul, 2001  Peer-reviewed
    We systematically investigated the molecular defects causing a primary LPL deficiency in a Japanese male infant (patient DI) with fasting hyperchylomicronemia (type I hyperlipoproteinemia) and in his parents, Patient DI had neither LPL activity nor immunoreactive LPL mass in the pre- and post-heparin plasma, The patient was a compound heterozygote for novel mutations consisting of a G-to-T transversion at the first nucleotide of exon 5 [+1 position of 3 ' acceptor splice site (3 ' -ass) of intron 4] and a T-to-C transition in the invariant GT at position +2 of the 5 ' donor splice site (5 ' -dss) of intron 8 (Int8/5 ' -dss/t(+2)c). The G-to-T transversion, although affecting the 11 nucleotide of the 3 ' -consensus acceptor splice site, resulted in a substitution of Gly(154) to Val (G154V; G (G) under bar (716)C(-->)G (T) under barC). The mutant G154V LPL expressed in COS-1 cells was catalytically inactive and hardly released from the cells by heparin, The 1nt8/5 ' -dss/t(+2)c mutation inactivated the authentic 5 ' splice site of intron 8 and led to the utilization of a cryptic 5 ' -dss in exon 8 as an alternative splice site 133 basepairs upstream from the authentic splice site, thereby causing joining of a part of exon 8 to exon 9 with skipping of a 134-bp fragment of exon 8 and intron 8.jlr These additional mutations in the consensus sequences of the 3 ' and 5 ' splice sites might be useful for better understanding the factors that are involved in splice site selection in vivo.
  • Y Ikeda, K Goji, A Takagi
    CLINICAL SCIENCE, 99(6) 569-578, Dec, 2000  Peer-reviewed
    We systematically investigated the molecular defects resulting in primary lipoprotein lipase (LPL) deficiency in a Japanese male infant thereafter called 'the patient') with severe fasting hypertriglyceridaemia (type I hyperlipoproteinaemia). The primary LPL deficiency was diagnosed on the basis of the findings that no LPL activity was detected in post-heparin plasma (PHP) and that the immunoreactive LPL mass in PHP was less than 2% of the control level. The patient was a compound heterozygote for a novel missense mutation (G(568)GA --> AGA/Gly(105) --> Arg; G105R) in exon 3 and a missense mutation (GAC(867) --> GAG/Asp(204) --> Glu; D204E) in exon 5 of the LPL gene. The biological significance of both missense mutations was examined by an in vitro study of the expression of the mutant G105R LPL cDNA and D204E LPL cDNA in COS-I cells. Both mutant LPLs were catalytically inactive and were barely released by heparin from the expressing COS-I cells. These findings explain the failure to detect LPL activity and immunoreactive LPL mass in the patient's PHP. The G105R allele could be detected by digestion with the BsmAI restriction enzyme, and the D204E allele by digestion with Hindi. The patient inherited the G105R allele from his mother and the D204E allele from his father. His parents were heterozygotes for the corresponding mutant allele, but normolipidaemic. The novel G105R missense mutation could not be detected by conventional analysis of single-strand conformation polymorphism, but it was identified by extensive sequencing of the entire exons and their flanking regions in the LPL gene.
  • A Mori, A Takagi, Y Ikeda, A Yamamoto
    CLINICAL BIOCHEMISTRY, 33(4) 323-327, Jun, 2000  Peer-reviewed
  • M Nishimura, Y Ohkaru, H Ishii, N Sunahara, A Takagi, Y Ikeda
    JOURNAL OF IMMUNOLOGICAL METHODS, 235(1-2) 41-51, Feb, 2000  Peer-reviewed
    We have developed a direct sandwich-enzyme-linked immunosorbent assay (ELISA) for quantification of the hepatic triglyceride lipase (HTGL) immunoreactive mass in human plasma. This direct sandwich-ELISA uses a combination of two distinct monoclonal antibodies (MAbs), which recognize different epitopes on the HTGL molecule: a horseradish peroxidase (HRP)-labeled anti-human HTGL MAb (2(4)F12C12) as an enzyme-linked MAb, and an anti-human HTGL MAb (1(11)A3H3) coated on a microtiter plate as a solid-phase MAb, Purified human post-heparin plasma (PHP)-HTGL was used as the standard material. The detection range of the sandwich-ELISA was 40-800 ng of HTGL protein per mi of plasma. The intra- and inter-assay coefficients of variation were less than 2.0% and 2.3%, respectively. The recovery tests resulted in variation only between 97.7% and 103.5%. No significant assay interference was caused by a high concentration of triglyceride, hemoglobin, bilirubin, uric acid, or creatinine. The reliability of the HTGL mass values obtained with the direct sandwich-ELISA was assessed by comparison with the HTGL mass values determined by our earlier one-step sandwich-enzyme immunoassay (EIA), The two sets of values showed a highly significant correlation (r = +0.952, n = 64). Strong correlation (r = +0.959, n = 50) was also found between the HTGL masses with the direct sandwich-ELISA and the HTGL activities determined with a selective immunoinactivation assay. The HTGL mass concentrations in PHP from 64 healthy subjects were 1916 +/- 841 ng/ml by the direct sandwich-ELISA and 1925 +/- 785 ng/ml (mean +/- standard deviation (SD)) by the one-step sandwich-EIA. The present direct sandwich-ELISA permits rapid identification of certain HTGL abnormalities in PHP samples from patients with hypertriglyceridemia or diseases such as hypothyroidism or renal failure, which affect HTGL. (C) 2000 Elsevier Science B.V. All rights reserved.
  • Takagi, A, Ikeda, Y, Takeda, E, Shinozuka, Yamamoto, A
    Biochimica et Biophysica Acta, 1502(3) 433-446, 2000  Peer-reviewedLead author
    We have systematically investigated the molecular defects resulting in a primary lipoprotein lipase (LPL) deficiency in a Japanese male infant (proband SEI) with fasting hyperchylomicronemia. Neither LPL activity nor immunoreactive LPL mass was detected in pre- or postheparin plasma from proband SH. DNA sequence analysis of the LPL gene of proband SH revealed homozygosity For a novel missense mutation of F270L (Phe(270) --> Leu/TTT1065 --> TTG)z in exon 6. The function of the mutant F270L LPL was determined by both biochemical and immunocytochemical studies. In vitro expression experiments on the mutant F270L LPL cDNA in COS-1 cells demonstrated that the mutant LPL protein was synthesized as a catalytically inactive form and its total amount was almost equal to that of the normal LPL. Moreover, the synthesized mutant LPL was non-releasable by heparin because the intracellular transport of the mutant LPL to the cell surface - by which normal LPL becomes heparin-releasable - was impaired due to the abnormal structure of the mutant LPL protein. These findings explain the failure to detect LPL activities and masses in pre- and postheparin plasma of the proband. The mutant F270L allele generated an XcmI restriction enzyme site in exon 6 of the LPL gene. The carrier status of F270L in the proband's family members was examined by digestion with XcmI. The proband was ascertained to be homozygous for the F270L mutation and his parents and sister were all heterozygous. The LPL activities and masses of the parents and the sister (carriers) were half or less than half of the control values. Regarding the phenotype of the carriers, the mother with a sign of hyperinsulinemia manifested hypertriglyceridemia (type IV hyperlipoproteinemia), whereas the healthy father and the sister were normolipidemic. Hyperinsulinemia may be a strong determinant of hypertriglyceridemia in subjects with heterozygous LPL deficiency. (C) 2000 Elsevier Science B.V. All rights reserved.
  • Y Ashida, A Takagi, Y Ikeda
    SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, 59(8) 663-669, Dec, 1999  Peer-reviewed
    A reliable method for detection of small-sized low-density lipoprotein (LDL) produced during transient hypertriglyceridaemia induced by ingestion of fat is described. Electrophoresis using a commercial non-denaturing 2.0-16% polyacrylamide gradient gel is commonly utilized to determine the size of LDL particles, but it failed to detect a minor change in LDL size induced during postprandial hypertriglyceridaemia. Detection of small-sized LDL was achieved by adjusting the polyacrylamide concentration. Electrophoresis using a nondenaturing 1.5-10% polyacrylamide gradient gel gave the best resolution for detecting small-sized LDL induced during postprandial hypertriglyceridaemia. This improved method may be useful for elucidating the underlying mechanism of production of small-sized LDL (small dense LDL) in subjects with chronic hypertriglyceridaemia.
  • A Takagi, Y Ikeda, K Tachi, T Shinozuka, A Yamamoto
    CLINICA CHIMICA ACTA, 285(1-2) 143-154, Jul, 1999  Peer-reviewedLead author
    We herein report a case of a 5-month-old Japanese female (patient AN) with fasting hyperchylomicronemia due to a primary lipoprotein lipase (LPL) deficiency. Patient AN was compound heterozygous for a missense mutation (GG(818)G-->GAG/Gly(188) -->Glu; G188E) in exon 5 and a nonsense mutation (TGG(1401)-->TGA/Trp(382) --> Stop; W382X) in exon 8 of the LPL gene. This resulted in less than 10% of the control levels for both the LPL activity and immunoreactive LPL mass in the postheparin plasma. A G188E mutation was thus identified for the first time in a Japanese, and the haplotype of this G188E allele was different from that of the G188E alleles identified in other ethnic groups. This additional mutation might be useful for early diagnosis of LPL gene aberrations in Japanese patients with fasting hyperchylomicronemia. (C) 1999 Elsevier Science B.V. All rights reserved.
  • H Kimura, Y Ohkaru, K Katoh, H Ishii, N Sunahara, A Takagi, Y Ikeda
    CLINICAL BIOCHEMISTRY, 32(1) 15-23, Feb, 1999  Peer-reviewed
    Objective: The purpose of this study was to develop and evaluate a direct sandwich enzyme-linked immunosorbent assay (ELISA) for quantification of the lipoprotein lipase (LPL) immunoreactive mass in human plasma using monoclonal antibodies (MAbs) directed against LPL purified from human postheparin plasma (PHP). Design: The DNA fragment containing exon 1 through 7 of the LD-H gene were amplified by PCR and directly sequenced. Total RNA was prepared from venous blood and the proportion of LD-H cDNA to total LD cDNA was semiquantified. Methods and results: The direct sandwich-ELISA was performed using a combination of two distinct types of MAbs that recognize different epitopes on the LPL molecule. The immunoreactive mass of human LPL was specifically measured using a horseradish peroxidase-labeled anti-human LPL MAb [1(1)D2B2] as an enzyme-linked MAb, and an anti-human LPL MAb [2(10)F8F9] coated on a polystyrene microtiter plate as a solid-phase MAb. Purified human PHP-LPL was used as a standard material. The detection range of the sandwich-ELISA was 3.6-460 ng of LPL protein per mt of plasma. The intra- and interassay coefficients of variation were less than 5.9% and 3.3%, respectively. The validity of this method was additionally assured by the recovery test, which resulted in the Variation only between 97.5% and 105.1%, and also by the interference test, which resulted in noninterference of LPL assay with a high concentration of triglyceride, hemoglobin, bilirubin, uric acid, or creatinine. To assess the reliability of the LPL mass values obtained with the direct sandwich-ELISA, they were compared with LPL mass values determined by the one-step sandwich-EIA (MARKIT-F LPL EIA kit) previously established by us. This comparison showed a highly significant correlation (r = +0.990) between the two sets of values. The LPL mass concentrations in PHP from 33 healthy subjects were 267 +/- 53 and 257 +/- 59 ng/mL (mean +/- SD), respectively. Conclusion: The present direct sandwich-ELISA is useful for rapidly identifying certain abnormalities of LPL in PHP samples from patients with hypertriglyceridemia. Copyright (C) 1999 The Canadian society of clinical Chemists.
  • 中田裕生, 世羅康彦, 兵藤純夫, 浜本和子, 西 美和, 高木敦子, 池田康行
    小児科臨床, 52 907-910, 1999  Peer-reviewed
  • S Suga, N Tamasawa, Kinpara, I, H Murakami, N Kasai, T Onuma, Y Ikeda, A Takagi, T Suda
    JOURNAL OF INTERNAL MEDICINE, 243(4) 317-321, Apr, 1998  Peer-reviewed
    WE herein report a case of a 40-year-old Japanese woman (patient IT) with a history of recurrent aggravation of hypertriglyceridaemia, pancreatitis and miscarriages in three previous pregnancies. However, strict dietary intervention was applied during a fourth pregnancy. As a result, acute pancreatitis was avoided, and the patient gave birth to a healthy infant. In patient IT, the underlying etiology of the recurrent aggravation of hypertriglyceridaemia during pregnancy was a lipoprotein lipase (LPL) gene aberration, She was homozygous for LPL deficiency due to a nonsense mutation (TGG(1401) --> TGA/Trp(382) --> Stop) in exon 8 of the LPL gene, which resulted in the absence of LPL activity and immunoreactive LPL mass, Our findings indicate that, in LPL deficiency pregnancy seriously exacerbates hypertriglyceridaemia and increases the risk of acute pancreatitis, which endangers both the mother and fetus. Early diagnosis of LPL deficiency and appropriate management thereof are essential for normal childbirth.
  • A Mori, A Takagi, Y Ikeda, A Yamamoto
    CLINICAL BIOCHEMISTRY, 30(4) 315-324, Jun, 1997  Peer-reviewed
    Objective: The purpose of this study was to develop an improved method of direct DNA sequencing, which makes it possible to identify heterozygous mutations of the lipoprotein lipase (LPL) gene in order to understand the underlying genetic disorder of type IV hyperlipoproteinemia. Methods and Results: The direct sequencing method was improved by devising primers for amplifying the LPL gene and for sequencing DNA amplified by the polymerase chain reaction (PCR), since the reported base sequences of the introns flanking exons of the LPL gene were limited to 40 bases. Improvement was achieved by attaching nine additional bases to both the PCR amplification primer and sequencing primer, and by optimizing the Tm Value of the sequencing primers by adjusting the sequence of the nine extra bases. Use of the sequencing primers having suitable Tm values (48 degrees C similar to 58 degrees C) made it possible to reduce nonspecific bands on the sequence ladder pattern and to identify heterozygous mutation sites in LPL gene exons 5 and 6 as model cases. Conclusion: Our improved direct sequencing method is useful for identifying heterozygous mutation sites in human LPL gene exons and splicing consensus regions.
  • A Takagi, Y Ikeda, A Mori, Y Ashida, A Yamamoto
    MOLECULAR AND CELLULAR PROBES, 11(2) 163-166, Apr, 1997  Peer-reviewedLead author
  • A Takagi, Y Ikeda, A Mori, Y Ashida, A Yamamoto
    MOLECULAR AND CELLULAR PROBES, 10(4) 313-314, Aug, 1996  Peer-reviewedLead author
  • A Mori, A Takagi, Y Ikeda, Y Ashida, A Yamamoto
    MOLECULAR AND CELLULAR PROBES, 10(4) 309-311, Aug, 1996  Peer-reviewed
  • Zenta Tsutsumi, Yasuyuki Ikeda, Atsuko Takagi, Motoo Tsushima, Kazuya Higashino, Akira Yamamoto
    Nippon Shokakibyo Gakkai Zasshi, 92(6) 951-959, 1995  Peer-reviewed
    We systematically investigated the effect of lipoprotein lipase (LPL) and alcohol intake on the development of primary type IV hyperlipoproteinemia which is manifestated as a moderate increase in serum triglycerides as a consequence of an elevated very low density lipoprotein concentration and a normal level of serum cholesterol. Twenty six type IV hyperlipoproteinemic and 28 normolipidemic males underwent measurement of the immunoreactive LPL mass in postheparin plasma (30 unit of heparin/kg of body weight) and were interviewed as to their alcohol consumption. They were divided into 4 groups, with a LPL mass of 150ng/ml (20 percentile of the normal range) and an alcohol intake of 33g/day as the borderlines. All subjects with LPL mass less than 150ng/ml and alcohol intake of more than 33g/day were prone to manifest type IV hyperlipoproteinemia. One of the subjects in this group showed a recovery to normolipidemic state after cessation of alcohol intake. Subject whose LPL mass value was more than 180ng/ml (50 percentile of the normal range) were not susceptible to type IV hyperlipoproteinemia even though they drank alcohol more than 33g/day. © 1995, The Japanese Society of Gastroenterology. All rights reserved.
  • A TAKAGI, Y IKEDA, A MORI, Z TSUTSUMI, K OIDA, T NAKAI, A YAMAMOTO
    JOURNAL OF LIPID RESEARCH, 35(11) 2008-2018, Nov, 1994  Peer-reviewedLead author
    We investigated measures for identification of heterozygous lipoprotein lipase (LPL) deficiency in unrelated subjects with primary type IV hyperlipoproteinemia in order to acquire a helpful clue for understanding the correlation between hypertriglyceridemia and the status of being a heterozygous carrier of an LPL gene variant. Identification of heterozygous LPL deficiency was performed by monitoring the immunoreactive LPL mass in postheparin plasma (PHP) using our developed sandwich-enzyme immunoassay technique for first screening. Then, in subjects found to have half or less than half of the control LPL mass value in PHP, the polymerase chain reaction-single strand conformation polymorphism method was used to detect LPL gene aberrations as a second screening. This approach was evaluated as being useful as it succeeded in identifying a subject (proband KD) with heterozygous LPL deficiency. The mutation in the LPL gene of proband KD was newly characterized as a nucleotide C-972 to A transversion in exon 6, resulting in substitution of a premature termination codon (TGA) for Cys(239) (TGC). This nonsense mutation, designated as LPL(obama), creates an MboI restriction site and eliminates an HgiAI restriction site, and this allows rapid screening of subjects with type IV as well as type I hyperlipoproteinemia for the mutation. The homozygous state for the LPL(obama) allele resulted in neither detectable LPL activity nor immunoreactive LPL mass in PHP, and this was seen in two of proband KD's siblings.
  • 朝倉由加利, 都島基夫, 西大條靖子, 原納 優, 池田康行, 高木敦子, 山本 章
    動脈硬化, 22 179-186, 1994  Peer-reviewed
  • A TAKAGI, Y IKEDA, Z TSUTSUMI, T SHOJI, A YAMAMOTO
    JOURNAL OF CLINICAL INVESTIGATION, 89(2) 581-591, Feb, 1992  Peer-reviewedLead author
    We have systematically investigated a genetic defect resulting in a primary lipoprotein lipase (LPL) deficiency in a proband TN and his affected brother SN, both manifesting familial hyperchylomicronemia. Neither LPL activity nor immunoreactive LPL mass was detected in postheparin plasma from the two patients. Immunocytochemical and biosynthetic studies on the proband's monocyte-derived macrophages with rabbit antihuman LPL antiserum revealed that no immunochemically detectable LPL protein was found in either the cells or culture medium, whereas LPL having a molecular mass of 61 kD was detected in normal cells. No detectable LPL mRNA was identified from poly(A)+RNA of the proband's macrophages by Northern blot analysis, and grossly visible LPL gene rearrangement was not observed by Southern blot analysis. Sequence analysis of polymerase chain reaction-amplified LPL gene exons detected one base deletion of G (first position of Ala221) at base 916 in exon 5 which leads to a premature termination by a frameshift. This mutation, designated as LPL(Arita) and resulting in the loss of an AluI restriction enzyme site, was newly identified. We further analyzed the LPL gene from the two patients and their family members by digestion with AluI. Both patients were homozygous for LPL(Arita) allele, while their spouses did not have this mutation. As genetically expected, their children were all heterozygous for LPL(Arita). We conclude that primary LPL deficiency in the proband was caused by a lack of enzyme synthesis due to the absence of LPL mRNA resulting from one base deletion of G in exon 5, and that heterozygous LPL(Arita) deficient subjects show almost half value of control LPL mass.
  • 高木 敦子, 池田 康行
    日本農芸化学会誌, 66(7) 1120-1121, 1992  Lead author
  • A TAKAGI, Y IKEDA, A YAMAMOTO
    NUCLEIC ACIDS RESEARCH, 18(21) 6436-6436, Nov, 1990  Peer-reviewedLead author
  • Y IKEDA, A TAKAGI, Y OHKARU, K NOGI, T IWANAGA, S KUROOKA, A YAMAMOTO
    JOURNAL OF LIPID RESEARCH, 31(10) 1911-1924, Oct, 1990  Peer-reviewed
  • Yasuyuki Ikeda, Atsuko Takagi, Akira Yamamoto
    Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, 1003(3) 254-269, Jun 28, 1989  Peer-reviewed
    Lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) were purified to homogeneity from human postheparin plasma. Molecular, catalytic and immunological properties of the purified enzymes were investigated. The native molecular weights of LPL and HTGL were 67 200 and 65 500, respectively, by gel chromatography. The subunit molecular weights of LPL and HTGL were 60 600 and 64 600, respectively, suggesting that these enzymes are catalytically active in a monomeric form. In addition, the purified LPL and HTGL each gave a single protein band when they were detected as glycoproteins with a probe of concanavalin A. The purified enzyme preparations were free of detectable antithrombin III by Western blot analysis. Catalytic properties of the purified enzymes were examined using triolein-gum arabic emulsion and triolein particles stabilized with phospholipid monolayer as substrates. LPL catalyzed the complete hydrolysis of triolein to free oleate and monooleate in the presence of apolipoprotein C-II. Apparent Km values for triolein and apoliproprotein C-II were 1.0 mM and 0.6 μM, and Vmax was 40.7 mmol/h per mg. HTGL hydrolyzed triolein substrate at a rate much slower than LPL, and produced mainly free oleate with little monooleate. Apparent Km and Vmax values were 2.5 mM and 16.1 mmol/h per mg, respectively. Polyclonal antibodies were developed against the purified LPL and HTGL. The purity and specificity of these antisera were ascertained by immunotitration, Ouchterlony double diffusion and Western blot analyses. The anti-human LPL and anti-human HTGL antiserum specifically reacted with the corresponding either native or denaturated enzyme, indicating that two enzymes were immunologically distinct. We developed an assay system for LPL and HTGL in human PHP by selective immunoprecipitation of each enzyme with the corresponding antiserum. © 1989.
  • 高木敦子, 池田康行, 山本 章
    動脈硬化, 15 1565-1568, 1988  Peer-reviewedLead author
  • A TAKAGI, EN CHUA, C BOONCHIRD, S HARASHIMA, Y OSHIMA
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 23(2) 123-129, 1985  Peer-reviewedLead author
  • A TAKAGI, S HARASHIMA, Y OSHIMA
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 49(1) 244-246, 1985  Peer-reviewedLead author
  • S HARASHIMA, A TAKAGI, Y OSHIMA
    MOLECULAR AND CELLULAR BIOLOGY, 4(4) 771-778, 1984  Peer-reviewed
  • Takagi A, Harashima S, Oshima Y
    Applied Enviromental Microbiology, 45 1034-1040, 1983  Peer-reviewedLead author

Misc.

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 199

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